| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
calcium ion |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
cytokinesis |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
fura-2 |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
probenecid |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
tip growth |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
坂口,修一
Kyoko,Shibuya
いいだ,ひでとし
あんらく,やすひろ
すずき,たかひと
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The dimorphic fungusCandida albicans has both a yeast form and a hyphal form. When yeast-form cells were starved and then transferred to aN-acetylglucosamine medium, the formation of true hyphae from the unbudded yeast-form cells was induced. Removal of Ca2+ from the medium with EGTA inhibited hyphal formation by 50%, resulting in only thin and short hyphae. Externally applied excess Ca2+ (>10-2M) also affected the hyphal formation, resulting in formation of pseudohyphae. This effect required a high concentration of Ca2+ but was Ca2+-specific. Deprivation of Ca2+ also inhibited yeast-form growth. Interestingly, such cells had abnormally wide bud necks and became defective in cell separation. To measure cytosolic free Ca2+, fura-2 was introduced into hyphal cells by electroporation. Its normal value was estimated to be about 100 nM. The electroporation caused transient elevation of cytosolic free Ca2+ concentration and transient cessation of hyphal growth. There was a close correlation between the timing of recovery of Ca2+ concentration and that of the resumption of hyphal growth. Our results demonstrate the importance of extracellular and intracellular free Ca2+ for the growth ofC. albicans. |
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言語 |
en |
| 書誌情報 |
en : Mycoscience
巻 38,
号 2,
p. 215-225,
発行日 1997-05-26
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| 出版者 |
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出版者 |
Springer-Verlag |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1340-3540 |
| 書誌レコードID |
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識別子タイプ |
NCID |
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関連識別子 |
AA11003436 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1007/BF02460856 |
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関連名称 |
10.1007/BF02460856 |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
AA11632817V38pp215-225.pdf
