{"created":"2024-03-22T07:20:53.992072+00:00","id":2002226,"links":{},"metadata":{"_buckets":{"deposit":"8d2d2623-9225-4200-95ba-7dab70851ce9"},"_deposit":{"created_by":8,"id":"2002226","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2002226"},"status":"published"},"_oai":{"id":"oai:nara-wu.repo.nii.ac.jp:02002226","sets":["1708505779733:1708506225397:1708506244269"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-05-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"225","bibliographicPageStart":"215","bibliographicVolumeNumber":"38","bibliographic_titles":[{"bibliographic_title":"Mycoscience","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The dimorphic fungusCandida albicans has both a yeast form and a hyphal form. When yeast-form cells were starved and then transferred to aN-acetylglucosamine medium, the formation of true hyphae from the unbudded yeast-form cells was induced. Removal of Ca2+ from the medium with EGTA inhibited hyphal formation by 50%, resulting in only thin and short hyphae. Externally applied excess Ca2+ (>10-2M) also affected the hyphal formation, resulting in formation of pseudohyphae. This effect required a high concentration of Ca2+ but was Ca2+-specific. Deprivation of Ca2+ also inhibited yeast-form growth. Interestingly, such cells had abnormally wide bud necks and became defective in cell separation. To measure cytosolic free Ca2+, fura-2 was introduced into hyphal cells by electroporation. Its normal value was estimated to be about 100 nM. The electroporation caused transient elevation of cytosolic free Ca2+ concentration and transient cessation of hyphal growth. There was a close correlation between the timing of recovery of Ca2+ concentration and that of the resumption of hyphal growth. Our results demonstrate the importance of extracellular and intracellular free Ca2+ for the growth ofC. albicans.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer-Verlag","subitem_publisher_language":"en"}]},"item_10001_relation_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"AA11003436","subitem_relation_type_select":"NCID"}}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/BF02460856"}],"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/BF02460856","subitem_relation_type_select":"DOI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1340-3540","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakaguchi,Shuichi","creatorNameLang":"en"},{"creatorName":"さかぐち,しゅういち","creatorNameLang":"ja-Kana"},{"creatorName":"坂口,修一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"1000020221997","nameIdentifierScheme":"kaken2","nameIdentifierURI":"https://nrid.nii.ac.jp/ja/nrid/1000020221997"}]},{"creatorNames":[{"creatorName":"Kyoko,Shibuya","creatorNameLang":"en"},{"creatorName":"しぶや,きょうこ","creatorNameLang":"ja-Kana"}]},{"creatorNames":[{"creatorName":"Hidetoshi,Iida","creatorNameLang":"en"},{"creatorName":"いいだ,ひでとし","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yasuhiro,Anraku","creatorNameLang":"en"},{"creatorName":"あんらく,やすひろ","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Suzuki,Takahito","creatorNameLang":"en"},{"creatorName":"すずき,たかひと","creatorNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-11-24"}],"filename":"AA11632817V38pp215-225.pdf","format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://nara-wu.repo.nii.ac.jp/record/2002226/files/AA11632817V38pp215-225.pdf"},"version_id":"522eabe0-d548-4c52-a184-8cca74fdc9b2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"calcium ion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytokinesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fura-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"probenecid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tip growth","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"8","path":["1708506244269"],"publish_date":"2017-11-24","publish_status":"0","recid":"2002226","relation_version_is_last":true,"title":["Roles of Ca2+ in hyphal and yeast-form growth in Candida albicans. Growth regulation by altered extracellular and intracellular free Ca2+ concentrations"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-08-05T02:48:12.395456+00:00"}