| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Applicability of environmental DNA metabarcoding for the hyporheic zone of a stream bed |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
direct sampling |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
eDNA metabarcoding |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hyporheos |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
macroinvertebrates |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
non-invasive investigation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
riverine ecosystem |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
Tanaka Aki
片野 泉
Doi Hideyuki
Iguchi Mami
Koike Shohei
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Hyporheic zones play a crucial role in ecological processes primarily orchestrated by hyporheic organisms, known as hyporheos. Our understanding of riverine biodiversity, encompassing hyporheic zones, remains limited. Therefore, the development of a method for the facile and accurate detection of hyporheic communities is imperative. Thus, we evaluated the applicability of environmental DNA (eDNA) metabarcoding surveys in the hyporheic zone in a small stream in Japan. We conducted simultaneous direct sampling and eDNA surveys at two different locations (head and tail of a rapid and a bar) and during two different seasons (less and more disturbed periods). Subsequently, we compared the outcomes of these sampling methods, locations, and seasons. Through eDNA metabarcoding targeting the cytochrome-c oxidase subunit I (COI) regions, we successfully identified a diverse array of hyporheic and benthic taxa in all samples. Remarkably, the performance of eDNA metabarcoding was comparable to the identification of directly sampled benthos and hyporheos. We observed a 62%–78% concordance between direct sampling and eDNA metabarcoding. Notably, eDNA metabarcoding revealed a higher number of hyporheic taxa compared to direct sampling. Moreover, the relative detectability of hyporheos by eDNA metabarcoding differed between the seasons, unlike direct sampling. Our findings underscore the importance of conducting both eDNA surveys and direct sampling to comprehensively assess the composition of the stream community while accounting for seasonal variations. This study demonstrates the utility of eDNA metabarcoding as a non-destructive approach for investigating the hyporheic zone, enabling a more effective assessment of riverine biodiversity. |
|
言語 |
en |
| 書誌情報 |
en : Environmental DNA
巻 5,
号 6,
p. 1667-1678,
発行日 2023
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| 出版者 |
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出版者 |
Wiley |
|
言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2637-4943 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1002/edn3.484 |
| 権利 |
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|
権利情報 |
© The Authors |
|
言語 |
en |
https://doi.org/10.1002/edn3.484
