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Escherichia coli persisters in biofilm can perform horizontal gene transfer by transformation
http://hdl.handle.net/10935/0002006056
http://hdl.handle.net/10935/0002006056abd6a429-8a67-4136-8f09-40b049900d32
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
https://doi.org/10.1016/j.bbrc.2024.150549
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| アイテムタイプ | default_学術雑誌論文 / Journal Article(1) | |||||||||||||
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| タイトル | ||||||||||||||
| タイトル | Escherichia coli persisters in biofilm can perform horizontal gene transfer by transformation | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Persisters | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Horizontal gene transfer | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Transformation | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Biofilms | |||||||||||||
| キーワード | ||||||||||||||
| 言語 | en | |||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Plasmid transfer | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | metadata only access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||||
| 著者 |
Nasu Tsubasa
× Nasu Tsubasa
× 前田 純夫
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Persisters represent a subset of cells that exhibit transient tolerance to antimicrobials. These persisters can withstand sudden exposure to antimicrobials, even as the majority of normal cells perish. In this study, we have demonstrated the capacity of ampicillin-tolerant and alkali-tolerant persisters to execute horizontal gene transfer via in situ transformation within biofilms. Air–solid biofilms, comprising two Escherichia coli populations each with a distinct plasmid, were formed on agar media. They were treated with lethal doses of ampicillin or NaOH for 24 h, followed by a 1-min glass-ball roll. This process led to a high frequency of horizontal plasmid transfer (10−7–10−6 per cell) from dead cells to surviving persisters within the biofilms. Plasmid transfer was DNase-sensitive and also occurred by adding purified plasmid DNA to plasmid-free biofilms, demonstrating a transformation mechanism. This marks the first evidence of persisters’ novel ability for horizontal gene transfer, via transformation. | |||||||||||||
| 言語 | en | |||||||||||||
| 書誌情報 |
en : Biochemical and Biophysical Research Communications 巻 738, p. 150549, 発行日 2024-08-01 |
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| 出版者 | ||||||||||||||
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE | |||||||||||||
| 言語 | en | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0006-291X | |||||||||||||
| ISBN | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | 10.1016/j.bbrc.2024.150549 | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | © The Authors | |||||||||||||
| 言語 | en | |||||||||||||
