| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Deuterium Labeling of Isoaspartic and Isoglutamic Acids for Mass Spectrometry Analysis |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hydrogen isotopes |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Ions |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
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主題 |
Labeling |
| キーワード |
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|
言語 |
en |
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主題Scheme |
Other |
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主題 |
Monomers |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Peptides and proteins |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
中澤 隆
Masaru Miyagi
Evan Kiesel
Kelao Neumbo
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Isoaspartic acid (isoAsp) is a common protein modification that spontaneously arises from asparagine or aspartic acid and has been linked to various diseases and health conditions. However, current methods for identifying isoAsp sites in proteins often suffer from ambiguity and have not gained widespread adoption. We developed a novel method that exclusively labels isoAsp with deuterium. This method capitalizes on the unique structural characteristics of isoAsp residues, which possess a free α-carboxyl group and can form an oxazolone ring. Once the oxazolone ring forms, it facilitates racemization at the Cα-position, incorporating a deuteron from a D2O solvent. The sites of deuterium-incorporated isoAsp in proteins can be unequivocally determined by comparing the precursor and product ion masses of the peptides from proteins reacted in H2O and D2O. The effectiveness of this method has been demonstrated through its application to model proteins lysozyme and rituximab. Furthermore, we have confirmed that the isoAsp deuterium-labeling reaction efficiently labels both l- and d-isoAsp without distinction, as well as isoglutamic acid (isoGlu), for which no effective detection methods currently exist. |
|
言語 |
en |
| 書誌情報 |
en : Analytical Chemistry
巻 96,
号 7,
p. 3077-3086,
発行日 2024
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| 出版者 |
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出版者 |
American Chemical Society |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1520-6882 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1021/acs.analchem.3c05194 |
| 権利 |
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権利情報 |
© American Chemical Society |
|
言語 |
en |
https://doi.org/10.1021/acs.analchem.3c05194
