| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Cellular neuroscience |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Genetic vectors |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Neural circuits |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
犬束 歩
前嶋 翔
Mizoguchi Hiroyuki
金子 涼輔
Nomura Rei
高浪 景子
坂本 浩隆
尾仲 達史
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Transgenic animals expressing fluorescent proteins are widely used to label specific cells and proteins. By using a split Cre recombinase fused with mCherry-binding nanobodies or designed ankyrin repeat proteins, we created Cre recombinase dependent on red fluorescent protein (RFP) (Cre-DOR). Functional binding units for monomeric RFPs are different from those for polymeric RFPs. We confirmed selective target RFP-dependent gene expression in the mouse cerebral cortex using stereotaxic injection of adeno-associated virus vectors. In estrogen receptor-beta (Esr2)-mRFP1 mice and gastrin-releasing peptide receptor (Grpr)-mRFP1 rats, we confirmed that Cre-DOR can be used for selective tracing of the neural projection from RFP-expressing specific neurons. Cellular localization of RFPs affects recombination efficiency of Cre-DOR, and light and chemical-induced nuclear translocation of an RFP-fused protein can modulate Cre-DOR efficiency. Our results provide a method for manipulating gene expression in specific cells expressing RFPs and expand the repertory of nanobody-based genetic tools. |
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言語 |
en |
| 書誌情報 |
en : Communications Biology
巻 5,
号 1,
p. 979
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| 出版者 |
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出版者 |
Springer Nature |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2399-3642 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1038/s42003-022-03944-2 |
| 権利 |
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権利情報 |
© Springer Nature Limited |
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言語 |
en |
https://doi.org/10.1038/s42003-022-03944-2
