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Method for Measuring Cervical Vagus Nerve Activity in Conscious and Freely Moving Rats
http://hdl.handle.net/10935/0002005917
http://hdl.handle.net/10935/0002005917cbbea12e-282b-499c-931b-ad76e49c6b93
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
https://doi.org/10.1096/fasebj.2022.36.s1.r4360
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| アイテムタイプ | default_学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| タイトル | ||||||||||||||||||
| タイトル | Method for Measuring Cervical Vagus Nerve Activity in Conscious and Freely Moving Rats | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | metadata only access | |||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||||||||
| 著者 |
三木 健寿
× 三木 健寿× 池亀 静香
× 吉本 光佐
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | The vagus nerve innervates most of the organs in the thoracic and abdominal cavity and plays a central role in inter-organ networks through afferent and efferent vagal nerve activity. However, few attempts have been made to measure vagal nerve activity in the conscious state; thus, the nature of vagal nerve activity remains unclear. In this study, we attempted to develop a method to measure cervical vagal nerve activity in conscious, freely moving rats. Using male Wistar rats, the right cervical vagus nerve was dissected approximately 1 mm under anesthesia, and a bipolar electrode made of twisted stainless-steel wire was hooked onto the vagus nerve and fixed with silicone gel. A microcatheter for administering a long-acting local anesthetic (levobupivacaine hydrochloride 0.75%) was placed at least 5 mm peripherally from the electrode. Measurements were recorded under conscious and free-living conditions. The right cervical vagal nerve activity (rcVNA) showed continuous activity of approximately 20 μV at peak-to-peak potential. The rcVNA was reduced by approximately 80% after administering 30 μL of levobupivacaine through the microcatheter. This allowed us to block the afferent VNA and selectively measure the efferent VNA. Using this method, we observed that efferent rcVNA decreased during REM sleep compared with NREM sleep. Moreover, we succeeded in measuring rcVNA and renal SNA simultaneously and continuously in conscious rats. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : The FASEB Journal 巻 36, 号 S1, 発行日 2022 |
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| 出版者 | ||||||||||||||||||
| 出版者 | Federation of American Societies for Experimental Biology | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||
| 収録物識別子 | 1530-6860 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.1096/fasebj.2022.36.S1.R4360 | |||||||||||||||||
| 権利 | ||||||||||||||||||
| 権利情報 | © Federation of American Societies for Experimental Biology (FASEB) | |||||||||||||||||
| 言語 | en | |||||||||||||||||
