| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Estradiol replacement improves high‐fat diet‐induced insulin resistance in ovariectomized rats |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
Akt2 |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
AS160 |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
estradiol replacement |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
high-fat diet |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
insulin resistance |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ovariectomized rat |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
中木 (横田) 直子
Omoto Sayo
Tazumi Shoko
Kawakami Mizuho
鷹股 亮
森本 恵子
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The role of 17β-estradiol (E2) in high-fat diet (HFD)-induced alteration of the protein kinase B (Akt) signaling pathway in ovariectomized (OVX) rats is unclear. Therefore, we examined whether chronic estrogen replacement restores HFD-induced impairment in insulin sensitivity by its effects concomitant with alterations in the Akt isoform 2 (Akt2) and Akt substrate of 160 kDa (AS160) phosphorylation in muscles of OVX rats. Nine-week-old female Wistar rats underwent ovariectomy under anesthesia; after 4 weeks, subcutaneous implantation of either E2 or placebo (PL) pellets was performed, and HFD feeding was initiated. Intravenous glucose tolerance tests were performed to assess insulin sensitivity. Following insulin injection into rats’ portal vein, the liver and gastrocnemius muscle were dissected for insulin signaling analysis. We observed that HFD increased energy intake and body weight in the PL group; however, it was temporarily decreased in the E2 group. Adipose tissue accumulation was larger in HFD-fed rats than in normal chow diet (NCD)-fed rats in the PL group; however, this difference was not observed in the E2 group. HFD reduced insulin sensitivity in the PL group only. In vivo insulin stimulation increased Akt2 phosphorylation in the muscles of NCD-fed rats in both groups. In contrast, HFD affected insulin-stimulated phosphorylation of Akt2 and AS160 in the muscles of rats in the PL group but not in the E2 group. Our data suggest that E2 replacement improves HFD-induced insulin resistance, and this effect is accompanied by the alterations in the Akt2 and AS160 phosphorylation in insulin-stimulated muscles of OVX rats. |
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言語 |
en |
| 書誌情報 |
en : Physiological Reports
巻 10,
号 5,
発行日 2022-03-02
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| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2051-817X |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.14814/phy2.15193 |
| 権利 |
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権利情報 |
© The Authors |
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言語 |
en |
https://doi.org/10.14814/phy2.15193
