| アイテムタイプ |
default_学術雑誌論文 / Journal Article(1) |
| タイトル |
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タイトル |
Characterization of the expression of gastrin‐releasing peptide and its receptor in the trigeminal and spinal somatosensory systems of Japanese macaque monkeys: Insight into humans |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
gastrin-releasing peptide |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
gastrin-releasing peptide receptor |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
itch |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ligand derivative stain |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
macaque monkey |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
primates |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
trigeminal sensory system |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
高浪 景子
越智 拓海
小林 靖尚
長谷川 功紀
Ito Takashi
筒井 直昭
上田 康雅
Carstens Earl
坂本 竜哉
坂本 浩隆
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Gastrin-releasing peptide (GRP) and its receptor (GRPR) have been identified as itch mediators in the spinal and trigeminal somatosensory systems in rodents. In primates, there are few reports of GRP/GRPR expression or function in the spinal sensory system and virtually nothing is known in the trigeminal system. The aim of the present study was to characterize GRP and GRPR in the trigeminal and spinal somatosensory system of Japanese macaque monkeys (Macaca fuscata). cDNA encoding GRP was isolated from the macaque dorsal root ganglion (DRG) and exhibited an amino acid sequence that was highly conserved among mammals and especially in primates. Immunohistochemical analysis demonstrated that GRP was expressed mainly in the small-sized trigeminal ganglion and DRG in adult macaque monkeys. Densely stained GRP-immunoreactive (ir) fibers were observed in superficial layers of the spinal trigeminal nucleus caudalis (Sp5C) and the spinal cord. In contrast, GRP-ir fibers were rarely observed in the principal sensory trigeminal nucleus and oral and interpolar divisions of the spinal trigeminal nucleus. cDNA cloning, in situ hybridization, and Western blot revealed substantial expression of GRPR mRNA and GRPR protein in the macaque spinal dorsal horn and Sp5C. Our Western ligand blot and ligand derivative stain for GRPR revealed that GRP directly bound in the macaque Sp5C and spinal dorsal horn as reported in rodents. Finally, GRP-ir fibers were also detected in the human spinal dorsal horn. The spinal and trigeminal itch neural circuits labeled with GRP and GRPR appear to function also in primates. |
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言語 |
en |
| 書誌情報 |
en : Journal of Comparative Neurology
巻 530,
号 16,
p. 2804-2819,
発行日 2022-09-14
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| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1096-9861 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
10.1002/cne.25376 |
| 権利 |
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権利情報 |
© The Authors. |
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言語 |
en |
https://doi.org/10.1002/cne.25376
